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0 %) was higher than Caucasians, lower than Iranians and Arabs while comparable to those reported among Indians of Mumbai city. In absence or unavailability of antisera particularly for low frequency alleles like In , such PCR based method would be extremely helpful to prepare rare donor registry by screening blood donors' at large scale. Red cells of In positive donors can be used as in-house reagent red cells for screening and identification of corresponding antibody. In absence or unavailability of antisera particularly for low frequ