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Effect sizes for significant differences were medium to large (Cohen's d 0.26-0.67). Alcohol- and cannabis-involved sexual activity tend to be overreported on retrospective surveys, and preliminary findings suggest that these recall biases may vary by gender. Researchers interested in the co-occurrence of substance use and sexual activity should be aware of this potential random error and consider how to reduce recall biases based on method of data collection. Alcohol- and cannabis-involved sexual activity tend to be overreported o

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Moreover, our transcriptomic data indicate a decrease in histone ubiquitination and methylation coinciding with the beginning of de novo DNA methylation. Decreases in H2AK119Ub and H3K27me3 were further confirmed by immunofluorescence in the male germ cells but were not consistent for all H3 methylation sites examined. Together, our data highlighted transient chromatin remodeling involving histone modifications during de novo DNA methylation. Further studies addressing how these dynamic changes in histone posttranslational modifications co