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Here, we describe a proof-of-principle method when it comes to production of isoprene from cellulosic biomass, the absolute most abundant polymer on the planet. We engineered the mesophilic prokaryote Clostridium cellulolyticum, that may degrade cellulosic biomass, to work well with the resulting sugar monomers as a feedstock when it comes to creation of isoprene. This was achieved by integrating the poplar gene encoding isoprene synthase. The existence of the enzyme was