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The observed substitution of positively charged His305 in HSA at subsite (-2) with an acidic Asp in LdAmy in the same position may explain the obtained energy reduction.Discharges of dyes-containing wastewater from industries have created global concern. Removal of these substances from aqueous solution is very important and essential. Magnetic chitosan coated with ZIF-8 was prepared successfully and used to eliminate cationic dye malachite green in various conditions. To find the effectiveness of ZIF-8 towards malachite green removal