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Finally, the film containing 4% ZIF-8 NPs (film 5) was chosen as the best sample due to it revealed appropriate mechanical properties, swelling, drug release and cell viability among all samples examined. This study reports on the exploitation of keratinous hydrolysate by keratinase enzymes to produce vitamin B-complex. Toward this end, keratinase enzyme was produced by Bacillus thuringiensis strain MT1, newly isolated from cattle-yard utilising donkey hairs. Scanning electron microscope (SEM) and Fourier transform infrared spectrophotom
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