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Uniform and sturdy gene manipulation is a must for effective assays. Nevertheless, neuronal cells are usually difficult-to-transfect cells with standard DNA/RNA transfection reagents. Therefore, virus-mediated gene delivery is a primary option for the research of gene functions in neurons. In this part, we shall explain the methods for lentivirus-mediated gene expression or knockdown in DRG neurons.The molecular players managing the axon deterioration pathway happen identified using in vitro experimental d
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