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Approximately 75% of xenobiotics are primarily eliminated through metabolism, and thus the accurate scaling of metabolic clearance is vital to successful drug development. Yet hepatic metabolic clearance, the major source of metabolism, still commonly underpredicts when scaled from in vitro to in vivo data. Over the past decades, several new cell culture settings have been investigated in order to improve hepatocyte functionalities using biophysics as a key component to restore aspect of the in vivo environment. Most of these studies have

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