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No provisions are made presently for giant-scale analysis or production of viable organisms containing recombinant DNA molecules that require BL4 containment at the laboratory scale. Level of physical containment is required for giant-scale research or production of viable organisms containing recombinant DNA molecules that require BL3 containment at the laboratory scale. Appendix K replaces portions of Appendix G when portions in excess of 10 liters of culture are involved in analysis or production. For large-scale research or manufacturing, 4 p