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In the following, the scientific emphasis (in general) has changed from the traditional fields of the first MECO to complexity and interdisciplinary themes as well.Many applications in biotechnology and medicine, among other disciplines, require the rapid enumeration of bacteria, preferably using miniaturized portable devices. Microfluidic technology is expected to solve this miniaturization issue. In the enumeration of bacteria in microfluidic devices, the technique of aligning bacteria in a single line prior to counting is the key to