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Results The proportion of MG1655/pTF2 of the total viable bacteria was significantly higher at high zinc concentrations (6 and 8mM) compared to low concentrations (0-4mM). The mRNA levels of blaCTX-M-1 in the two ESBL strains increased at increasing zinc concentrations and varied with the growth phase.Conclusion The growth of the inoculated CTX-M-1-encoding E. coli MG1655 strains and natural occurring coliforms was impacted differently when exposed to zinc oxide. The blaCTX-M-1 mRNA expression levels seemed to increase with increasing zi