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SEM images revealed more scattered exopolysaccharides in the biofilm after UHPH treatment compared to the control. Additionally, UHPH treatment resulted in planktonic cells having a greater shift to smaller cell size and a wider cell size distribution compared with detached-cells; this indicated a higher resistance of detached-cells to UHPH. This finding suggests that although UHPH has great potential application in food sterilization, the resistance of detached-cells cannot be ignored.In the last decades, mathematical models and model-