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Doxycycline is employed to reduce Wolbachia from all life stages of heartworm in order to prevent huge releases of this bacteria through the loss of the worms. Nevertheless, the dosage and extent currently recommended were extrapolated from the remedy for various other rickettsial attacks. Consequently, desire to was to study the dynamics of Wolbachia IgG antibodies in heartworm-infected puppies under adulticide treatment utilizing different dosages of doxycycline. Forty-nine heartworm-