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Swift and effective diagnosis of acute myocardial infarction (AMI) is critical to patient survival due to its serious life-threatening effects and increasing incidence. Creatine kinase MB (CK-M is one of the markers of AMI. In this work, we enabled a portable visual quantitative assay of CK-MB by incorporating target-responsive DNA hydrogel with a microfluidic chip. The CK-MB aptamer and the complementary short DNA strand were grafted onto the polyacrylamide strand separately and formed the hydrogel by base-paired linkage. Upon introd