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However, we lack a comprehensive comprehension of the way the physicochemical properties of microgels relate solely to their interaction with cells. Right here, we show that HEK293T cells occupy PNIPAM-based microgels on a second-to-minute time scale. Uptake prices are determined by microgel size and cross-linker content. Utilizing fluorescence confocal live-cell microscopy, we observe microgel uptake in real-time and explain cellular uptake kinetics. Experiments reveal that tiny and less cross-li

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