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In two thirds of sequenced reverted cultures, the concentrating on spacers were discovered to be specifically excised through the miniCRISPR array, suggesting a still hypothetical, but extremely active recombination system acting on the characteristics of CRISPR spacer arrays. Our outcomes indicate that CRISPR kind III - based silencing is a broadly appropriate device to examine in vivo features of crucial genetics in Sulfolobales which underlies a certain apparatus to pre
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