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TR1900028228). BMI- and BSA-adjusted HS could serve as indicators of physical health, and HSBSA may moderately reflect cardiorespiratory fitness levels in healthy young adults, particularly in males. Clinical trials registry site and number China Clinical Trial Center (ChiCTR1900028228). This pre-post study is aimed at determining the effects of masticatory muscle activity (masseter and temporalis) measured via sEMG between conventional, self-ligating, and ceramic bracket after six months of orthodontic treatment. A total of eighteen (18