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In addition, the limited availability of motile Lactobacillus isolates is among the significant hurdles for additional studies. To get insight into the ecology and habitat of this motile lactobacilli, we established a routinely relevant recognition way for motile lactobacilli utilizing PCR and subsequent discerning isolation in semi-solid MRS medium for the assortment of additional motile lactobacilli from animal feces. Results We used the PCR recognitio