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For this, the DAD V1M mutant (bDAD) ended up being overexpressed, purified, and reconstituted with various material ions. Kinetic assays assessing the game associated with the reconstituted enzyme plus the substrate- and product-dependences for the effect kinetics were carried out. The results from reconstitution of this apoprotein with a number of metal ions offer the requirement of an Fe3+ center for enzyme activity. Response prices showed easy saturation kinetics for DHA with values fo
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