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Comparison of predicted N2O gradients between water and air (ΔN2O) with those quantified from field measurements validates the predictive performance of the model and allow extending previous findings to large river networks including highly regulated rivers with cascade reservoirs and locks. Results show the major role played by the water column processes in contributing to N2O emissions in large rivers. Consequently, N2O productions along the UMR, characterized by regulated flows and large channel size, occur chiefly within this surfi