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Ultimately, the integration of 100% biochar and excess sludge can be used as a substitute for anaerobic sludge as an inoculum by giving similar overall performance.PLCzeta(ζ) initiates Ca2+ oscillations and egg activation at fertilization in mammals, but studies in mouse eggs fertilized by PLCζ knockout (KO) sperm imply that there is another slow acting factor causing Ca2+ release. Here, I propose a hypothesis for how this second sperm factor might cause Ca2+ oscillations in mouse eggs.Most of the anti-inflammatory drugs in clinical pra