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We report the de novo biosynthesis of alkyne-tagged polyketides by modular type I PKSs through starter unit engineering. Specifically, we use JamABC, a terminal alkyne biosynthetic machinery through the jamaicamide B biosynthetic path, in conjunction with representative modular PKSs. We prove that JamABC works as a trans loading system for designed type I PKSs to produce alkyne-tagged polyketides. In addition, the manufactu

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