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Inflammatory cytokines (IL-1α, GM-CSF and TNF-α), chemokines (IL-8, CCL2, CCL5 and CXCL1) and NF-κB activation (pNF-κB/NF-κ in HT29 cells increased after stimulation by KsAg. The immunofluorescence results of spores and lectins (concanavalin A and wheat germ agglutinin) recommended the necessity of Mincle in molecular recognition between Kudoa spores and abdominal cells. Practically, data for Mincle-Fc and KsAg binding affinity, CLEC4E mRNA phrase, Mincle immunofluoresce
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