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Taken together, our work actually provides in-depth information for PRRSV pathogenesis, which supports book opportunities for the improvement antiviral medicines and vaccines.IMPORTANCEPRRS, due to PRRSV, is an economically vital aspect in pig farming all over the world. As a lipid membrane-wrapped virus, merging of PRRSV envelope with number cell membrane is indispensible for viral infection. Nonetheless, there is deficiencies in knowledge on its membrane layer fusion. Here, we first e