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The present results not only provided new insights into the interaction mechanism of RXFP3 with agonists and antagonists, but also demonstrated usefulness of the NanoBiT-based homogenous binding assay to study the interaction mechanism of certain receptors with their ligands.Terror management theory proposes cultural worldview and self-esteem as two buffers against death anxiety. The neural mediators of these buffering effects, however, have not been fully understood. The present work investigated neural mediation mechanisms between sel