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Excessive endoplasmic reticulum (ER) stress in intestinal epithelial cells (IEC) may lead to impaired intestinal mucosal barrier function and then participate in the pathogenesis of ulcerative colitis (UC). Jianpi Qingchang decoction (JPQCD) has been shown to have protective effects on UC. However, further studies are needed to determine whether JPQCD regulates PERK/eIF2 /ATF4/CHOP pathways to play a role in treating UC. mice were randomly assigned into five groups control, model, low-dose JPQCD (JPQCD L), middle-dose JPQCD (JPQCD M), and