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Background Type 2 diabetes mellitus (T2DM) is an increasingly common disorder characterized by chronic hyperglycemia and marked dyslipidemia. This study evaluates the effect of vitamin D supplementation alone and in combination with glimepiride in streptozotocin-induced T2DM in rats. Materials and methods A total of 30 Wistar albino rats of either sex weighing 150-200 g were included in the study. The effect of oral administration of vitamin D was evaluated in streptozotocin-induced T2DM in rats. Blood glucose, serum insulin, serum HbA1