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5 mg EB was administered on Day -2). All groups received LA P4 on day 0 (D. Daily ultrasonography and blood collections were performed to assess uterine edema and estradiol and progesterone plasma concentrations, respectively. Endometrial biopsies were collected on Day 4 to evaluate P19 expression by RT-qPCR. There were no differences in P19 expression between groups LE2 and LE5. However, P19 expression was higher (P .1). In conclusion, the two EB administrations protocol is more feasible for preparing acyclic recipient mares at field conditions