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Most analyses of bispecific antibodies rely on liquid chromatography with mass spectrometry (LC-MS), that could miss recognition or becomes less quantitative if those types aren't physically separated. Here, we report a novel and painful and sensitive mixed mode dimensions exclusion chromatography (MM SEC) along with multiangle light-scattering (MALS) to evaluate different forms of bispecific IgG particles under native conditions. The method displayed great ability to split various