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nase-1/2 activity. Copyright © 2020 by The American Society for Pharmacology and Experimental Therapeutics.Little is known about the factors regulating carotenoid biosynthesis in flowers. Here, we characterize the Reduced Carotenoid Pigmentation 2 (RCP2) locus from two monkeyflower (Mimulus) species, the bumblebee-pollinated M. lewisii and hummingbird-pollinated M. verbenaceus. We show that loss-of-function mutations of RCP2 cause drastic down-regulation of the entire carotenoid biosynthetic pathway. We have identified the