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Four unknown stilbenoids, including one dimer, namely 4'-methoxy-scirpusin A (5) and three monomeric stilbene glycosides, namely piceatannol-3'-O-[2''-(3,5-dihydroxy-4-methoxybenzoyl)]-β-D-glucopyranoside (13), piceatannol-3'-O-(2''-galloyl)-β-D-glucopyranoside (14) and piceatannol-3'-O-(6″-p-coumaroyl)-β-D-glucopyranoside (16) together with 15 described compounds, were isolated from the ethyl acetate fraction of the ethanol extract of roots of Rheum lhasaense based on the guidance of the inhibitory effect on acetylcholinesterase. The s