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Red blood cell invasion by thePlasmodiumvivaxmerozoite requires interaction between the Duffy antigen receptor for chemokines (DARC) and the P. vivax Duffy-binding protein II (PvDBPII). Given that the disruption of this interaction preventsP. vivaxblood-stage infection, a PvDBP-based vaccine development has been well recognized. However, the polymorphic nature of PvDBPII prevents a strain transcending immune response and complicates attempts to design a vaccine. Twenty-three P. vivax clinical isolates collected from three areas of Ethiopi
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