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The mean inter-orifice distance between both canals at the level of the pulpal floor was 2.52±0.76 mm. The prevalence of MB2 canal in this Saudi sub-population was high. The prevalence decreased as the root canal approached the apical third. There was no correlation between the average distance and occurrence of canal joining. CBCT is useful in detecting and mapping the mesiobuccal root canal system, with a likelihood of enhancing the quality of root canal treatment. The prevalence of MB2 canal in this Saudi sub-population was high. The