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Ceramides (CERs) when you look at the stratum corneum (SC) are recognized to play a crucial role in deciding epidermis barrier function in puppies. We aimed to build up an in vitro tradition system that synthesized epidermal CER classes to higher comprehend the synthesis of CER courses in canine SC-. Canine keratinocyte cells (MSCEK) at proper confluency had been incubated with high Ca2+ (1.8 mM CaCl2) supplemented serum-free medium. Eight days po
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