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capsulatus strain lacking AnfA suggesting that AnfA is required for AnfHDGK production, but dispensable for biosynthesis of the iron-only cofactor and electron delivery to Fe-nitrogenase, pathways activated by NifA. These observations strengthen our model, in which the Fe-nitrogenase system in R. capsulatus is largely integrated into the Mo-nitrogenase system. © 2020 The Authors. MicrobiologyOpen published by John Wiley Sons Ltd.The human immunodeficiency virus (HIV) pandemic heavily affects sub-Saharan Africa. It is as