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Thus, we inferred that CsNRT2.4 was a key gene for N uptake in tea plant roots. This study provides new insights into the molecular mechanisms of tea plant responses to N resupply and reveals hub genes for improving nitrogen usage efficiency (NUE) in tea plants.Melatonin is a pleiotropic regulatory molecule in plants and is involved in regulating plant tolerance to drought stress. Here, we conducted transcriptomic and physiological analyses to identify metabolic processes associated with the enhanced tolerance of the melatonin-treated mai