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Then, we analyzed the differential phrase between NPWT and ST teams, looking at possible injury size effects. We found 12 differentially expressed miRNAs in pre-treatment contrast, including let-7f-2, while in post-treatment analysis we identified 28 miRNAs. The path enrichment analysis suggests that identified miRNAs may be involved with injury healing, specially through angiogenesis. Conclusion We found preliminary evidence that NPWT in T2DM clients with DFU impacts miRNA expression in plasma. Fu