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High mobility group package (HMG users are DNA binding proteins with varied features present across kingdoms. The device by which HMGBs with differing amount of HMG bins are able to complete comparable features, tend to be defectively grasped. More over, just how non-canonical DNAs are acquiesced by HMGB proteins is certainly not clear. To handle these, we carried out detailed biochemical and computational researches to characterize two HMGB members- Kinetoplast linked protein (KAP6) of Trypanosoma and tal