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Recent studies have revealed the extended nature of smooth muscle structures in the pelvic floor, revising the conventional understanding of the "perineal body." Our aim was to clarify the three-dimensional configuration and detailed histological properties of the smooth muscle structures in the region anterior to the rectum and anal canal in men. Four male cadavers were subjected to macroscopic and immunohistological examinations. The pelvis was dissected from the perineal side, as in the viewing angle during transperineal surgeries. Ser