https://www.selleckchem.com/pr....oducts/cid44216842.h
Structural analysis suggested that beneficial mutations introduced additional hydrophobic interactions and hydrogen bonds, contributing rigidification of the flexible loops and the increase of internal forces, hence increasing the thermostability and activity. 5 g DCW (dry cell weight) L-1KmAKRM9 completely reduced 350 g L-1t-butyl 6-cyano-(5R)-hydroxy-3-oxo-hexanoate ((5R)-1), within 3.7 h at 40 °C, yielding optically pure (3R,5R)-2 (d.e.p 99.5%) with a space-time yield (STY) of 1.82 kg L-1 d-1. Hence, KmAKRM9 is a robust bioc
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