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These results further illuminated the relationship between inflammation and inhibition of smooth muscle contraction under Se deficiency in the small intestine. The Ca2+ concentration was decreased, and RhoA phosphorylation and ROCK expression were also inhibited by Se deficiency. The results also showed that MLC protein phosphorylation decreased with Se deficiency. In conclusion, the present study indicated that inflammation under Se deficiency leads to the inhibition of smooth muscle contraction in the small intestine.A healthy diet is