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The aim of the present article was to analyze the phytochemical composition of EtOAc extract of Cirsium italicum (Savi) DC. and to determine the antioxidant activity of a new compound 1. The EtOAc extract of C. italicum was purified by different chromatographic methods. Compound 1 was isolated from the whole plant of C. italicum. Its structure was established on the basis of UV, IR, 1 D, 2 D NMR, and HRESI-MS methods and antioxidant activities of this compound were investigated using TEAC, FRAP, and CUPRAC assays. According to the