https://www.selleckchem.com/pr....oducts/ro5126766-ch5
Sperm DNA integrity has become one of the most discussed and promising biomarkers for the assessment of male fertility. However, an easy-to-apply method capable of estimating DNA fragmentation in the live fraction of spermatozoa has remained elusive, preventing this parameter from being fully applied in clinical settings. To validate a novel co-staining for the analysis of DNA fragmentation in membrane-intact spermatozoa. Normozoospermic semen samples were used to validate the co-staining consisting of acridine orange (AO) a
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