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To evaluate the effect of ionizing radiation and cariogenic biofilm challenge using two continuous flow models, normal and reduced salivary flow, on the development of initial root-dentin caries lesions. Microcosm biofilms were grown under two salivary flow rates (0.06 and 0.03mLmin ) and exposed to 5% sucrose (3 × daily, 0.25mLmin , 6min) dripped over non-irradiated and irradiated root-dentin blocks for up to 7days. The vibration modes of root dentin, matrix/mineral (M/M), and carbonate/mineral (C/M) ratios were evaluated by FTIR. The

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