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1, 1, 5 ng/mL), demonstrating the suitability of this method for large-scale epidemiological studies. We successfully applied it to rapidly analyze a total of 565 serum samples from prediabetic and healthy individuals in a nested case-control panel study. Oxylipin concentrations were quantified within a range similar to those of previously published articles. Application of this approach to both healthy and prediabetic subjects found that several circulating hydroxy PUFAs, including LTB4, 12-HEPE, 15(S)-HETE, and 17-HDHA, were