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The proposed principle can be readily applied to dielectrophoresis-based devices for biomedical sample preparation or diagnosis such as the separation of rare or infected cells from a blood sample. © 2020 WILEY-VCH Verlag GmbH Co. KGaA, Weinheim.RATIONALE The offline coupling of high-performance thin layer chromatography (HPTLC) with atmospheric sample analysis probe - Mass Spectrometry (ASAP-MS) was evaluated for the characterization of polymeric additives in gasoline. METHODS A protocol was developed to optimize the ion signal.