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The effective use of the resulting substrate ended up being performed by enrichment and SERS detection of E. coli. To this end, the spherical gold nanoparticle-embedded cellulose nanofibril substrate had been utilized as a scavenger for E. coli. Following the target micro-organisms E. coli were separated from the matrix via focused antibodies, the sandwich assay treatment ended up being completed using 5,5-dithiobis-(2-nitrobenzoic acid) (DTN-coated Au nanorod