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To assess knowledge, attitude and practice of health care professionals working in Abha primary health care (PHC) centers regarding standard precautions of infection control. This cross sectional study included 212 health care professionals in Abha PHC centers. An electronic questionnaire was constructed by the researchers and was used for data collection. It consisted of five parts, i.e., socio-demographic characteristics, knowledge questions about infection control and standard precautions, statements about attitude of participants