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Fish bile has been applied as a biomarker for environmental contamination for several decades, and several pollutants are known to be excreted in this matrix. With the advent of the proteomic field, however, the discovery of protein biomarkers of response to pollutants has become the highlight, and fish bile shows very high potential in this regard. A proteomic case study carried out in Southeastern Brazil with mullet bile indicates the importance of assessing bile colour, as different feeding statuses lead to differential proteomic profil