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There were no differences in cell viability, density of neurons or microglia. The densify of visible short branches of microglia in live imaging was higher in collagen membranes than PTFE membranes. Collagen membranes are suitable for live imaging of cellular dynamics in slice cultures using an inverted microscope. Collagen membranes are suitable for live imaging of cellular dynamics in slice cultures using an inverted microscope.Inducible degron systems are widely used to specifically and rapidly deplete proteins of interest in cell lin

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In MS WT mice, WAS induced VHS for up to 4 weeks. In cKO however, visceral pain perception returned to basal level by week 4. The development of VHS in WT mice was associated with significant upregulation of spinal ephrin-B2 and EphB1 mRNA expression or protein levels in the TNBS model and upregulation of spinal ephrin-B2 protein in the MS model. No changes were observed in cKO mice. VHS was not associated with persistent intestinal inflammation. CONCLUSIONS AND INFERENCES Overall, our data indicate that the ephrin-B2/EphB1 spinal s