https://www.selleckchem.com/pr....oducts/e7766-diammon
Label-based functional studies of biomolecules in their native environment require labeling reactions inside living cells. In cell spin labeling using alkyne-azide click chemistry with a Gd3+-DOTAM-azide complex is shown to provide high spin label stability and narrow EPR lines for EPR spectroscopic detection of a spin labeled protein in living cells at ambient temperatures.In living cells, the exceptionally high local concentration of macromolecules, or "locally crowded environment," could affect many aspects of cellular
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